M. Sc., Ph. D.

• Molecular Cell Biology of macromolecular interactions.
• Transcriptomics, proteomics and metabolomics of Giardia lamblia under Oxidative stress.
• Pathogenesis of parasitic protozoa Biochemistry and oxidative stress biology of parasitic protozoa and drug development against parasitic infection.
• Molecular epidemiology of enteric parasites.

• Junior Research Fellow of CSIR-UGC NET from 2009 to 2012 in the project entitled "STUDIES ON OXIDATIVE STRESS MANAGEMENT IN Giardia lamblia" at National Institute of Cholera & Enteric Diseases, Kolkata.
• Senior Research Fellow of CSIR-UGC NET from 2013 to 2014 in the project entitled "STUDIES ON OXIDATIVE STRESS MANAGEMENT IN Giardia lamblia" at National Institute of Cholera & Enteric Diseases, Kolkata.
• Senior Research Fellow of ICMR from 2014 to 2015 in the project entitled "STUDIES ON THE MODULATION OF SIGNALLING PATHWAYS IN Giardia lamblia DURING OXIDATIVE STRESS FOR ITS SURVIVAL" at National Institute of Cholera & Enteric Diseases, Kolkata.
• Training program under JICA 3rd country training program on Molecular Parasitology at Division of Parasitology, in National Institute of Infectious Diseases, Japan in 2016.
• 14th February, 2017 started working as Assistant Professor and Head at Department of Biochemistry, Vivekananda College (Under C.U.), Thakurpukur, Kolkata, WB, India.
• Working with Molecular parasitology laboratory, NICED, ICMR, Kolkata-10 in collaboration with Dr. Sandipan Ganguly.

6 Years as Head of the Department of Biochemistry.

7 years teaching experience along with technical skills.

TECHNICAL SKILLS:

 Identification of different parasites by microscopy.
 Culture of the parasites like Giardia (Portland 1) and Entamoeba (HM1) under in vitro condition.
 Viability assays of parasites (Trypan blue, MTT).
 Isolation of nucleic acids from parasites, polymerase chain reaction (PCR), RNA extraction, cDNA preparation and reverse transcriptase polymerase chain reaction, immunoblotting, gel electrophoresis.
 Apoptosis study by DNA ladder assay, Hoechest stain, Tunnel assay, PI stain and Flow Cytometry analysis. Histology, Phase contrast microscopy, Fluorescence microscopy, preparation of tissue sample for transmission electron microscopy.
 Separation of cytosolic and microsomal fractions of parasites, assay of malondialdehyde (MDA) & conjugated diene, Glutathione-S- tranferases assay, Superoxide dismutase assay from cytosolic fraction. Reactive Oxygen Species (ROS) measurement from parasites by NBT study.
 Microarray analysis of gene expression.
 Cloning, expression studies and polyclonal antibody preparation. Localization studies by confocal microscopy.
 Spectrophotometer, Spectrofluorometer and Sequencing analysis.
 2D gel electrophoresis and Mass spectrometry.
 Animal handling.

Guest Lecturer in the PG-Department of Zoology, Vivekananda College.

• Invitee Member of UGBOS, Biochemistry, CU

Poster Presentation
1. Dibyendu Raj, Sandipan Ganguly. Influence of pyruvate on oxidative stress regulation in the amitochondriate Giardia lamblia. Seminar of Society for Biological Chemists (SBC), Bhubaneswar, December 2014.

2. Dibyendu Raj, Sandipan Ganguly. Pyruvate Induces ROS generation in Giardia under metronidazole stress. Organized by Lady Brabourne College, Kolkata.

3. Dibyendu Raj and Sandipan Ganguly. Influence of pyruvate on oxidative stress regulation in the microaerophilic Giardia lamblia. ASCODD, 2022.

Abstract Publication
4. Ghosh E, Ghosh A, Mukherjee AK, Raj Dibyendu, Chowdhury P, Karmakar S and Ganguly S. Oxidative stress regulation in Giardia lamblia. B.C. Guha National Symposium for Young Investigator, 2010.

5. Mukherjee AK, Raj Dibyendu, Ganguly S. Genotyping of Giardia lamblia in Kolkata; 22nd National Congress of Parasitology 2010 – Indian Society of Parasitology, Zoological Society of India and University of Kalyani.

6. Dibyendu Raj and Sandipan Ganguly. Role of pyruvate in oxidative stress relief in Giardia. Annual Asian-African Research Forum organized by the Japan Initiative for Global Research Network on Infectious Diseases held in Sendai, Japan during January 20-22, 2014.

7. Avik K Mukherjee, Koushik Das, Punam Chowdhury, Dibyendu Raj, Sumallya Karmakar, Tomoyoshi Nozaki and Sandipan Ganguly. “Surveillance and genetic diversity of enteric parasites in Kolkata” International Congress of Parasitology” held in Mexico City, Mexico during August 10-14, 2014.

8. Dibyendu Raj and Sandipan Ganguly. “Studies on the oxidative stress management in the amitochodriate Giardia lamblia” in 16th Asian Conference on Diarrhoeal Disease and Nutrition organized by ICMR-National Institute of Cholera & Enteric Diseases, Kolkata during November 11-13, 2022.

Book Chapters:

1. Sandipan Ganguly, Dibyendu Raj. Giardia and Giardiasis: An overview on recent developments. The Handbook of Microbial Bioresource; (eds V.K Gupta et al.) CAB International 2016.
2. Dibyendu Raj, Sanjib Kumar Sardar, Sandipan Ganguly. Giardia, giardiasis and its zoonotic transmission. Advances in Medico-Veterinary Parasitology: An Indian Perspective. 2018. 81-8653-580-2, pp 511-529.
3. Dibyendu Raj, Sandipan Ganguly. Oxidative Stress Regulation in Giardia lamblia. Oxidative Stress in Microbial Diseases. 2019. 978-981-13-8763-0, pp 281-296.

Articles in Peer Reviewed International Journals:

List of Publication:

1. Dibyendu Raj, Tomoyoshi Nozaki, Shanta Dutta, Sandipan Ganguly. Metronidazole Induces the Metazoan Death of Giardia Trophozoites with the Aid of Pyruvate. 2024. International Journal of TROPICAL DISEASE & Health, 45: 1, 13-24.

2. Sandipan Ganguly, Sharad Barkataki, Sumallya Karmakar, Prerna Sanga, K. Boopathi, K. Kanagasabai, P. Kamaraj, Punam Chowdhury, Rituparna Sarkar, Dibyendu Raj et. al., Epidemiology of soil-transmitted helminth infections among primary school children in the States of Chhattisgarh, Telangana, and Tripura, India, 2015-16. 2022. American Journal of Tropical Medicine & Hygiene. 107: 1, 122-129.

3. Dibyendu Raj et. al., Pyruvate protects Giardia trophozoites from cysteine-ascorbate deprived medium induced cytotoxicity.2018. Korean J Parasitol. 56(1): 1–9.

4. Sandipan Ganguly, Sharad Barkataki, Sumallya Karmakar, Prerna Sanga, K. Boopathi, K. Kanagasabai, P. Kamaraj, Punam Chowdhury, Rituparna Sarkar, Dibyendu Raj et. al., High prevalence of soil-transmitted helminth infections among primary school children, Uttar Pradesh, India, 2015. 2017; Infectious Diseases of Poverty, (6), 139.

5. N.Dutta⁠⁎, D. Raj, N.Biswas, M.Mallick, S.Omesh. Nanoparticle assisted activity optimization and characterization of a bacterial phytase immobilized on single layer grapheme oxide. Biocatalysis and Agricultural Biotechnology. 240-247, 2017.

6. Dibyendu Raj, Prasanta Saini, Tomoyshi Nozaki and Sandipan Ganguly. Involvement of pyruvate on oxidative stress management in the microaerophilic protozoan parasite Giardia lamblia.2015. Int J Adv Res, 3: 1148-1166.

7. Raj Dibyendu1, Ghosh E, Mukherjee A. K., Nozaki T. and Ganguly S. Differential Gene Expression in Giardia lamblia under Oxidative Stress: Significance in Eukaryotic Evolution. Gene 535: 131–139; 2014.

8. Karmakar S1, Raj Dibyendu1 and Ganguly S. Identification of the N-terminal Glycine-arginine Rich (GAR) Domain in Giardia lamblia Fibrillarin and Evidence of its Essentiality for snoRNA Binding. Int J Trop Dis Health; 3. 318-327; 2013.

9. Ghosh A., Karmakar S., Mukherjee. A. K., Raj. Dibyendu., Das. K., Sarkar. S., Nozaki. T. and Ganguly. S. The spliceosomal protein SnRNP F binds to both U3 and U14 class of snoRNA in Giardia lamblia. Glob J Biol Agr Health Sc, Vol 2(3): 178-184, 2013.

10. Mukherjee A. K., Chowdhury. P., Das. K., Raj. Dibyendu., Karmakar. S., and Ganguly. S., Helminth burden among school going children of southern Bengal, India: A survey report. Glob J Biol Agr Health Sc, Vol 2(3): 189-191. 2013.

11. M Khalid Ijaz, Kaisar A Talukder, Mohammad Aslam, Rashidul Haque, Sandipan Ganguly, Ishrat J Azmi, Md Shawkat Hossain, Avik Kumar Mukherjee, Dibyendu Raj, Ijaz Ahmed, Jabeen Kamal, Joseph R Rubino, Alam Nur-E-Kamal., Natural contamination of human hands with enteric parasites in Indian Subcontinent. World J Clin Infect Dis; 25; 3(2): 13-19. 2013.

12. Mukherjee, A. K., Karmakar S, Raj Dibyendu and Ganguly S. Multi-locus genotyping of Giardia duodenalis reveals high percentage of mixed assemblages within a limited geographical boundary. Brit Microbiol Res. J. 190-197. 2013.